Immunophenotyping gating strategy and morphological analysis for MDSC identification in peripheral blood of dogs.

<p>PBMCs from healthy dogs and dogs with cancer were stained for the myeloid marker CD11b, monocytic marker CD14 and MHC II. (A) Representative flow cytometric analysis of forward and side scatter and gated CD11b⁺CD14⁻MHCII⁻ cells from dogs with advanced or metastatic tumors compared to dogs with early stage non-metastatic tumors and healthy control dogs. Plots are representative of dog with advanced metastatic hemangiosarcoma (top), early stage bladder transitional cell carcinoma (middle) and a healthy dog. (B) FACS sorted CD11b⁺CD14⁻MHCII⁻ cells were stained with diff-quick for cell morphology evaluation. A representative example of polymorphonuclear granulocyte morphology of CD11b⁺CD14⁻MHCII⁻ cells is shown at 63× magnification.