Comparison of tumor cell labeling between topical application of GB119 and systemic administration of GB123.

<p>(<b>a–d</b>) Representative unmixed false colored maps of pixel intensity of coronal 2 mm sections (<b>a</b>,<b>b</b>) and dorsal surface tumors (<b>c</b>,<b>d</b>) comparing the pattern of activation of topical GB119 (left side) and systemic GB123 (right side). (<b>e,f</b>) H&E coronal sections demonstrating tumor mass. (<b>g–j</b>) Merged fluorescent microscopy of adjacent sections showing labeling in the center (<b>g,h</b>) and edge (<b>I,j</b>) of the tumor. Sections were immunostained with anti-human vimentin antibody to reveal the brain tumor xenograft (false colored green), covalently bound Cy5 (false colored red). Yellow represents co-localized staining. (<b>k & i</b>) H&E sections demonstrating tumor cells that migrated away from the main tumor mass (outlined) via the ventricular system (<b>k</b>) or along the cortical meninges (<b>i</b>). Boxed regions indicate invading cells (<b>m</b> & <b>n</b>). Merged images of boxed regions from panels k & i showing labeling of invading tumor cells. Vimentin positive tumor cells (false colored green) and Cy5 cells (false colored red) reveals that invading cells were identified by topical GB119 (yellow cells) (<b>m</b>) but not by systemic GB123 (<b>n</b>). DAPI staining for cellular orientation was also included in panels <b>m,n</b>. Scale bar, 1 mm (<b>e,f</b>), 500 µm (<b>k,l</b>), and 50 µm (<b>g–j, m, n</b>).