Identification of genes regulating intracellular protein traffic and Golgi structure.

<p>(<b>A</b>) Putative mammalian orthologs and functional domains of candidate genes considered for further characterization. (<b>B</b>) S2 cells transiently transfected with pMT-Ren were cultured for 5 days with the indicated dsRNAs and treated as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033665#pone-0033665-g002" target="_blank">Figure 2F</a>. The bars represent the mean medium <i>Renilla</i> activity normalized by the lysate firefly activity ± SD. (<b>C</b>) S2 cells were transfected with MannII-GFP and cultured with dsRNAs against the indicated genes for 5 days. MannII-GFP staining was quantified by counting the number of cells displaying a normal, fused, or fragmented MannII-GFP pattern (see examples in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033665#pone.0033665.s005" target="_blank">Figure S5</a>), expressed as a percentage of the total cells counted (100–200 cells per treatment). (<b>D–I</b>) S2 cells were cultured with dsRNAs against the indicated genes for 5 days and processed for GMAP immunostaining (red). The control cells in D were treated with Ci dsRNA. Scale bar, 3 µm. (<b>D′–I′</b>) S2 cells treated with dsRNA for 5 days were analyzed by EM. Note the intact Golgi stacks in D′ (arrow head) compared to the disrupted Golgi structures (arrows) or elongated fragmented Golgi remnants (between brackets) in other panels. Scale bar, 0.5 µm.