Quantification of Parasite Stage Progression.

<p>HCT-8 coverslips were infected and stained with pan-specific antibody to quantify <i>C. parvum</i> infection. >300 parasites were counted on 15–30 frames for each of four replicates for each time point. The average for each of the 3 stages of parasite determined was converted into a percentage of the total parasite detected per time point. zoite = sporozoite/merozoite, trophozoite = all trophozoites (size of stained parasite was > zoite but trophozoite.