Effects of neurula-stage overexpression of P328L329del HNF1β mutation on larval development.

<p>Representative larvae of crossings between heterozygous HSPCre13 males and heterozygous 328del4 females. The F1 offspring were heat-shocked for two hours at 34°C at neurula (stage 19). A: Comparison of a larva with overexpressing the P328L329del HNF1β mutation with a non-transgenic control. Abbreviations: g, abnormal gut; e, reduced eye; t, abnormal tail; bracket: edema. Red fluorescence: HSPCre13 transgene. Blue fluorescence: 328del-4 transgene. HS: heat-shock treatment. The development of control larvae lacking either the effector or the activator transgene was not affected (data not shown). B: Analysis of the pronephric kidneys after overexpression of P328L329del HNF1β mutation at neurula stage. Mutant animals developed malformations of the eyes. Abbreviations: e, eye; pt, proximal tubules. White arrowheads indicate more distal parts of the pronephric kidney (pronephric duct). C and D: Quantitation of the average pronephric kidney (C) and eye sizes (D) in mutant and control animals. * = p<0.01 (Student's t-test). N refers to the number of single pronephri or eyes that were measured. Scale bar = 1 mm.