Presynaptic redistribution of the sumoylation machinery during neuronal maturation.

<p>Confocal images show the colocalisation (yellow) between the presynaptic marker Bassoon in green and in red, the sumoylation enzymes AoS1 and Ubc9 (<b>A</b>) or the desumoylases SENP1 and SENP6 (<b>B</b>) in 10 and 20 DIV cultured rat hippocampal neurons. Enlargement of hatched areas are also depicted. Scale bars, 20 µm. Quantification of presynaptic colocalisation was performed using the ImageJ software as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033757#s4" target="_blank">methods</a>. Histograms represent the relative presynaptic intensity of the sumoylation machinery and each value is the mean ± s.e.m. measured from 40 cells in four independent experiments. (<b>A</b>) Student's <i>t</i>-tests, *p<0.0001; n.s., not significant. (<b>B</b>) Student's <i>t</i>-tests, *p<0.05 and **p<0.001.