CDKIs are decreased in Hiwi–MSCs.

<p>(A) Western blot of indicated proteins reveals that p21 and p27 are decreased in Hiwi-MSCs. (B) Western blot of indicated proteins reveals that p21, p27 and p15 are increased in sh-Hiwi MFH cells that have been induced with doxycycline for 4 or 7 days. (C) IHC analysis of a human sarcoma TMA reveals a tight inverse correlation of p21, p27 and p15 expression (x-axes) to Hiwi expression (y-axis) (R² = 0.764; R² = 0.8679; R² = 0.7539, respectively) but no such correlation for p16 expression (x-axis) to Hiwi expression (y-axis) (R² = 0.5418). Ten cases of each subtype (present in triplicate) were scored from 0 to 2 blindly by sarcoma pathologists. Average scores for each case are plotted here.