Localization and dynamics of non muscle myosin II during morphogenesis and on application of external force.

<p>(A) Panel shows the images pre bleaching 0″ and post bleaching- 10″, 20″, 45″ and 120″ at different stages of <i>Drosophila</i> embryo development as well as different regions in the embryo. First row: Pre-blastoderm stage (Bownes stage -4), second row: Blastoderm stage (Bownes stage -5), third row: anterior region during slow phase of GBE (early Bownes stage -9), fourth row: posterior region during slow phase of GBE (early Bownes stage -9). Circle and arrow highlights the region where photo-bleaching is performed. Scale bar = 5 µm (B) Plot shows the FRAP curves of EGFP tagged non muscle myosin II at different stages and regions inside a live embryo. (Filled Triangle: stage 4, Filled square: anterior stage 9, Filled circle: posterior stage 9 and open circle: posterior stage 9 after application of force) (C) Bar graphs show fractional recovery after 110 sec of bleaching in all the above cases. Curves and bar graph shown here are average of 7,13, 18, 27 and 8 curves for preblastoderm, blastoderm, GBE-anterior, GBE- posterior and GBE-posterior after force respectively. Single plane at the dorsal side was imaged for FRAP.