Effect of soma constraints on axonal polarization.

<p>(A) Design of patterns DC, BmS, and DS; L1–L3 directions are indicated. (B) Immunolabelings of stage 3 neurons on DC, BmS and DS patterns: axon (tau staining, red), microtubules (tubulin staining, green) and nuclei (Hoechst staining, blue). The shape of the cells reflects the global organization of DC/DS patterns in a hexagonal network. Scale bar, 20 µm. (C) Results of axonal polarization, <i>i.e.</i> percentages of stage 3 neurons with their axon along L1–L3 directions (<i>n</i> = 194, 176 and 267 for the DC, BmS and DS patterns, respectively). *, significantly different from random (blue dotted line, 33.3% in each direction), <i>p</i><0.05.