Plasmid construction and generation of stable cells.

<p>(<b>A</b>) Schematic representation of miR-34a expression vector. (<b>B</b>) Relative miR-34a expression levels in three groups of SOSP-9607 cells (Blank, SOSP-9607 cells; control, stable SOSP-9607 cells transfected with pcDNA3.1; miR-34a, stable SOSP-9607 cells transfected with pcDNA-miR34a.). (<b>C</b>) Relative miR-34a expression levels in SAOS-2 cells transiently transfected with pcDNA3.1 and pcDNA-miR34a respectively. U24 small nucleolar RNA (RNU24) was used as an internal loading control to normalize the results. Data are presented as means±SD. *P<0.01 (n = 3) is considered as statistically significant.