Chromosomal distribution of EMF1 binding sites and H3K27me3 modified regions in WT and 3 mutants.

<p>(A) Top panel shows the comparison of H3K27me3 marked (black) and EMF1 binding (red) regions per 100 kb in WT on 5 chromosomes. Lower three panels show comparison of H3K27me3 in WT and three mutants (purple: <i>emf1</i>; blue: <i>emf2</i>; orange: <i>fie</i>). Arrows point at pericentromeric locations. (B) H3K27me3 and EMF1 binding patterns (EMF1_IP) on individual genes in WT and 3 mutants. <i>STK</i>: <i>SEEDSTICK</i>, <i>AGO5</i>: <i>ARGONAUTE5</i>, <i>CUC2</i>: <i>CUP-SHAPED COTYLEDON2</i>, <i>SUP</i>: <i>SUPERMAN</i>, <i>LOB</i>: <i>LATERAL ORGAN BOUNDARIES</i>. (C) WT and mutants grown at short day condition for 15 days. (D) The percentage of 7,751 H3K27 trimethylated genes showing reduced methylation in mutants. 44%, 54% and 84% of the H3K27me3 marked genes in WT show reduced methylation in <i>emf1</i>, <i>emf2</i> and <i>fie</i> mutants, respectively. The p-value is 10⁻¹³. (E) Venn diagram showing number of genes with reduced methylation that overlaps.