Separation of nucleoids and Z ring positioning in outgrown spores of <i>dnaB</i> (ts) strains.

<p>Refer to <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002561#pgen-1002561-g004" target="_blank">Figure 4A</a> for experimental design. (A) Separation of nucleoids in cells collected at 135 min after the start of germination, prior to induction of <i>ftsZ</i> expression. Columns read left to right; e.g. DnaB (ts), Min⁺Noc⁺: the mean cell length of the population was 3.8 µm; 43% of the population contained two separated nuceloids; cells with two nucleoids had a mean length of 4.0 µm; the mean internucleoid distance in cells with two nucleoids was 0.77 µm. (B) Z ring assembly and positioning in cells collected at 165 min after the start of germination, 30 min after induction of <i>ftsZ</i> expression. Columns read left to right: e.g. DnaB (ts), Min⁺Noc⁺: the mean cell length of the population was 6.8 µm; Z rings were present in 66% of cells, out of which 56% had one Z ring and two nucleoids. Of these cells with two nucleoids, 94% had a Z ring between two nucleoids and 6% had a Z ring between the nucleoid and the pole. SEM is standard error of the mean.