Bioluminescence quantification and lethality measurement in mice infected with <i>V. vulnificus</i> strains containing pHGJ1.

<p>(A) 5–6 weeks old C57BL/6 mice were infected with 1×10⁶ CFU of HG0905 (CMCP6<i>lux</i>) and isogenic mutants HG0906 (<i>ΔrtxA1</i>), HG0907 (<i>ΔvvhBA</i>) or HG0908 (<i>ΔrtxA1vvhBA</i>) strains i.g. as indicated. Representative images were acquired at 0, 4, 8, 12, 15, 19, 22.5 and 36 hr post infection, and the color scale of total flux represents the photons s⁻¹ cm⁻² sr⁻¹ for combined images is shown. (B–E) Survival curve and light emission from every individual mouse infected with HG0905 (B), HG0906 (C), HG0907 (D) and HG0908 (E) were measured. (F) Slopes (m) between onset and peak point from total flux of individual mice were measured. (G) <i>In vitro</i> growth curves of strains in LB broth at 30°C with shaking of HG0905, HG0906, HG0907 and HG0908 for OD₆₀₀.