Genetic interactions of heterochronic mutants.

1<p>All animals examined were homozygous for null alleles of the genes indicated and carry an integrated transgene <i>wIs78(scm::GFP; ajm-1::GFP)</i> to mark seam cells. All alleles are null.</p>2<p>Seam cell counts were performed on L4 animals except where indicated.</p>3<p>Alae formation was assessed in the early L4 stage.</p>4<p>Strains carrying the <i>let-7</i> mutation additionally contained a linked <i>unc-3</i> mutant allele. They were grown at 15°C to limit constitutive dauer formation that results from the <i>unc-3</i> mutation at higher temperatures in these backgrounds.</p>5<p>Seam cell fusion with no alae formation was observed in the other 85% of animals.</p><p>SEM, standard error of the mean; ND, not determined.