Properties of the murine anti-fHbp mAbs.

<p>All but one of the hybridomas were from CD-1 mice. The exception, JAR 65, was from a human fH transgenic BALB/c mouse <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034272#pone.0034272-Beernink6" target="_blank">[43]</a>.</p>*<p>fHbp mutants with amino acid substitutions at these positions lost (knocked-out) or gained (knocked-in) mAb binding <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034272#pone.0034272-Beernink3" target="_blank">[32]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034272#pone.0034272-Beernink4" target="_blank">[39]</a>.</p>**<p>The amide signals of Gly148, Arg149, and Ala174 showed chemical shifts by NMR upon binding with fHbp <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034272#pone.0034272-Scarselli1" target="_blank">[29]</a>.