NUP-1 depletion causes misregulation of metacyclic <i>VSG</i> (<i>MVSG</i>) genes in PCF cells.
- Kelly N. DuBois (176072)
- Sam Alsford (24967)
- Jennifer M. Holden (176080)
- Johanna Buisson (176084)
- Michal Swiderski (176090)
- Jean-Mathieu Bart (176093)
- Alexander V. Ratushny (102304)
- Yakun Wan (176097)
- Philippe Bastin (25974)
- J. David Barry (60385)
- Miguel Navarro (176103)
- David Horn (24969)
- John D. Aitchison (102330)
- Michael P. Rout (25373)
- Mark C. Field (129657)
Publication date
March 2012
DOIAbstract
<p>(A) eGFP reporter modification of the telomeric MVSG 1.22 locus in PCF cells. P, promoter; BSD, blasticidin<sup>R</sup> gene; Pol I, RNA polymerase l. (B) NUP-1 RNAi induction causes time-dependent increase in <i>MVSG</i> and eGFP expression. All values are fold expression compared to uninduced cells determined by qRT-PCR and normalized to β-tubulin. Clathrin, PLK, and ATPaseAF; RNA was isolated after manifestation of the proliferative/morphological phenotype.</p
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