CUP-1-GFP expressed in mammalian cells presented a vesicle-like localization pattern.

<p>Chimeric protein was transiently expressed in HEK293 cells and colocalization with organelle cell markers was evaluated by confocal microscopy in living cells. Colocalization channel was built from positive PDM values resulting from both channels pixels that were above the mean. (A) Colocalization of CUP-1-GFP and the lipophilic membrane marker FM4-64 (red) was observed in membrane areas as well as endosome-like structures (arrow heads). These endosomes-like structures also colocalized with the endosome marker rho-GTPase in cyan (B) and within lysosomes by the use of red-Lysotracker (C). (D) Immunostaining with human Golgin-97 antibody (red) showed a positive colocalization signal at the Golgi (arrow). R: Manders' Overlap coefficient. Scale bar 10 µm.