NUP-1 depletion causes increased expression of developmentally regulated genes in BSF cells.

<p>(A) Heatmap for microarray following NUP-1 knockdown in BSF cells. Of 8,110 genes, 62 were increased in relative expression greater than 2-fold. Yellow and blue represent upregulation and downregulation, respectively, and black no expression change. Gene accession numbers/annotation are given for the upregulated genes. (B) qRT-PCR quantitation of the expression of <i>EP</i> and <i>GPEET procyclin</i> genes (left panel), selected ES <i>VSG</i> genes (middle panel), and housekeeping genes (right panel) during NUP-1 RNAi. The expression level in control cells was set to one, and <i>y</i>-axis is the relative expression compared to control cells, normalized to β-tubulin.