MADM-Tet combines MADM with a binary expression system.

<p><b>A</b>) Schematic representation of MADM-Tet starting with the following genotype: <i>R26^TG/G-tTA2;Nestin-Cre^+/−;TRE-KZ^+/−</i>. Although all cells contain the <i>Nestin-Cre</i> and <i>TRE-KZ</i> transgenes, for simplicity they are displayed within the cells only when they are active. <b>B</b>) Confocal images of tissue sections stained with antibodies against GFP (green) and lacZ (red) from mice of the genotype indicated above. Note that the two markers exhibit different subcellular distribution: GFP labels whole cells including nuclei, whereas tau-lacZ is absent from the nuclei and labels the processes more strongly (an example of a red-only cell body is indicated by an arrowhead). Scale bars, left and middle panels: 50 µm, right panel: 25 µm.