Strong <i>BnAP2</i>-RNAi lines cause changes in seed development.

<p>A, Seeds of <i>BnAP2</i>-RNAi lines are less round in shape and larger in size than that of wild type (left, <i>BnAP2</i>-RNAi; right, wild type). B, The surface of the full <i>BnAP2</i>-RNAi seed is bumpy and radicels drill out from mature seeds. C, Abortive seeds in strong <i>BnAP2</i>-RNAi lines (arrow). D, Embryo of abortive seeds (left, <i>BnAP2</i>-RNAi; right, wild type). E, <i>BnAP2</i>-RNAi-26 plant seeds contain less protein than do wild-type seeds. Protein extracts from an equal number of wild-type and <i>BnAP2</i>-RNAi-26 seeds were fractionated on a 12% SDS polyacrylamide gel and stained. Molecular mass markers are shown to the left of the gel. Optical sections through developing <i>BnAP2</i>-RNAi (F–H) and wild-type seeds (I–K) at the indicated DAP. Seeds from three to five siliques at each time point were harvested, fixed and cleared and representative optical sections are shown.