Modular organizations of mycobacteriophage endolysins.

<p>Through combinations of 15 domains and sequence elements, 26 different organizations are observed in the 240 mycobacteriophage Lysin As. Representative Lysin A structures shown for each organization. N1–N5 (red and pink shades) and C1–C3 (purple shades) are N-terminal and C-terminal regions, respectively. Predicted catalytic functions include Ami-2A and Ami-2B (amidases, green and aquamarine), GH19 (glycoside hydrolase family 19, yellow), GH25 (glycoside hydrolase family 25, orange), M23 (M23 family peptidase, blue), and TG (lytic transglycosylase, cyan). Also identified are the predicted binding motifs PGBD (peptidoglycan binding domain, gray) and LGFP repeats (rose).