TGFβ expression is reduced in S100A9^−/− and TLR4^−/− animals.

<p>A. Quantitative real time RT-PCR analysis (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034207#s4" target="_blank">Materials and Methods</a>) of TGFβ RNA expression from CD11b⁺ cells (>90% pure by FACS analysis) from the spleen of C57BL/6, S100A9^−/− and TLR4^−/− animals in the absence of, or 14 days after inoculation, with 50,000 EL4 lymphoma cells subcutaneously. The mean expression from 4 separate experiments is shown where the expression in the C57BL/6 controls have been set to 1. B. ELISA measurements of TGFβ serum levels of C57BL/6 (filled circles), RAGE^−/− (filled triangles), S100A9^−/− (filled squares) and TLR4^−/− (filled diamonds) in the absence of, or 14 days after inoculation (open symbols), with 50,000 EL4 lymphoma cells subcutaneously. Statistical analysis using two-tailed t test *** p = 0.0008; * p = 0.039 and 0.0028, respectively. There was no statistical significant difference in TGFβ serum levels between EL4 inoculated C57BL/6 or RAGE^−/− mice.