Increasing vascular permeability enhances the accumulation of doxorubicin into the tumor.

<p>Doxorubicin was injected intravenously subsequent to administration of PBS or VEGF and its uptake at the tumor site in real time was estimated using its natural fluorescence. <b>A–B.</b> Representative images of doxorubicin uptake over time, tumors (green) and doxorubicin uptake (red) are shown. <b>C.</b> Heat map of doxorubicin uptake after 3 hours in control and VEGF-treated tumors. <b>D.</b> Graph showing relative uptake of doxorubicin in the tumor in the presence or absence of systemic VEGF treatment. <b>E.</b> Graph showing relative uptake of doxorubicin in the normal tissues distal to the tumor in the presence or absence of systemic VEGF treatment. N = 4 per treatment; data were analyzed by 2 way ANOVA.