<p>AmpR regulates many genes involved in biofilm formation (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034067#pone.0034067.s006" target="_blank">Table S6</a>) that is reflected in the tube biofilm assay monitored for 72 hours. The strains tested were wild type PAO1, PAOΔ<i>ampR</i>, PAOΔ<i>retS</i> (positive control), and PAOΔ<i>pel</i>Δ<i>psl</i>Δ<i>algD</i> (negative control). The inset, taken at 72 hours, demonstrates the superior biofilm formation capacity of PAOΔ<i>ampR</i> compared to PAO1. <i>p</i>-values comparing PAOΔ<i>ampR</i> with PAO1: 24 hrs- 0.002; 48 hrs- 0.03; 72 hrs- 0.007.</p
<p>(<b>A</b>) Stationary phase cultures of the indicated strains were diluted to an OD<sub>660</sub>...
<p>Microtiter plates were washed and stained with crystal violet and biofilms visualized in (A) and ...
<p>Relative biofilm formation ability (panels A, B) and susceptibility to the stress imposed by grow...
<p>AmpR positively regulates resistance to β-lactam antibiotics by upregulating expression of the <i...
<p>(A) Biofilm formation was assessed using the XTT reduction assay. The results are presented as th...
<p>Crystal violet assay of 1-day PittGG biofilms, formed in the presence of increasing amounts of am...
Stenotrophomonas maltophilia intrinsic resistance to β-lactams is mediated by two chromosomal β-lact...
PAO1, ΔgshA, ΔgshA::gshA, ΔgshB, ΔgshB::gshB and double ΔgshAΔgshB mutants were cultured in LB mediu...
<p>Confocal laser scanning microscopy (cLSM) of biofilms formed by NTHi strains 2019 and PittGG in t...
<p>A) Size-based separation of the cell-free culture supernatant from strain 69 isolates fractions t...
<p>(A) SK assays for <i>algR</i> deletion and overexpression strains. Loss of <i>algR</i> led to a s...
<p>Crystal violet assays of 1-day NTHi biofilms formed in the presence of amoxicillin or amoxicillin...
OBJECTIVES: In Pseudomonas aeruginosa, biofilm formation is controlled by a cell-to-cell signalling ...
Sessile bacterial communities which form on the solid surface or solid-liquid interface are known a...
<p>(A) The wild-type (CEC369) and <i>bcr1</i>Δ (JC1081) reference strains, <i>cbk1</i>Δ (JC1080), <i...
<p>(<b>A</b>) Stationary phase cultures of the indicated strains were diluted to an OD<sub>660</sub>...
<p>Microtiter plates were washed and stained with crystal violet and biofilms visualized in (A) and ...
<p>Relative biofilm formation ability (panels A, B) and susceptibility to the stress imposed by grow...
<p>AmpR positively regulates resistance to β-lactam antibiotics by upregulating expression of the <i...
<p>(A) Biofilm formation was assessed using the XTT reduction assay. The results are presented as th...
<p>Crystal violet assay of 1-day PittGG biofilms, formed in the presence of increasing amounts of am...
Stenotrophomonas maltophilia intrinsic resistance to β-lactams is mediated by two chromosomal β-lact...
PAO1, ΔgshA, ΔgshA::gshA, ΔgshB, ΔgshB::gshB and double ΔgshAΔgshB mutants were cultured in LB mediu...
<p>Confocal laser scanning microscopy (cLSM) of biofilms formed by NTHi strains 2019 and PittGG in t...
<p>A) Size-based separation of the cell-free culture supernatant from strain 69 isolates fractions t...
<p>(A) SK assays for <i>algR</i> deletion and overexpression strains. Loss of <i>algR</i> led to a s...
<p>Crystal violet assays of 1-day NTHi biofilms formed in the presence of amoxicillin or amoxicillin...
OBJECTIVES: In Pseudomonas aeruginosa, biofilm formation is controlled by a cell-to-cell signalling ...
Sessile bacterial communities which form on the solid surface or solid-liquid interface are known a...
<p>(A) The wild-type (CEC369) and <i>bcr1</i>Δ (JC1081) reference strains, <i>cbk1</i>Δ (JC1080), <i...
<p>(<b>A</b>) Stationary phase cultures of the indicated strains were diluted to an OD<sub>660</sub>...
<p>Microtiter plates were washed and stained with crystal violet and biofilms visualized in (A) and ...
<p>Relative biofilm formation ability (panels A, B) and susceptibility to the stress imposed by grow...