The RQR mutant accesses a much larger output space than wild-type MarA.

<p>Relative affinity of a MarA variant for a given site as determined by MITOMI (<a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002614#pgen-1002614-g004" target="_blank">Figure 4</a>) vs. the quantity of <i>tet</i> gene expressed. Expression levels were monitored by Q-PCR. We show data for wild type WQR MarA (orange diamonds) and the RQR mutant (blue squares). The transcriptional output was normalized with wild type MarA bound to its consensus site of ‘GCA’ = 1.