Stable Maintenance of pFCEN in the Parasites.

<p>(A) The GFP expression of the parasites transfected with pFCEN and pCon were monitored in the presence of the selective drug (upper panel) and at 8 days after the removal of the drug. The nuclei of the parasites were stained with Hoechst-33258. The scale bars indicate 10 µm. (B) The percentages of GFP-positive parasites in the absence of the drug were calculated as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0033326#s4" target="_blank">Materials and Methods</a>. The red and blue lines indicate the percentage of GFP-positive parasites transfected with pFCEN and pCon, respectively.