Immunolocalization of rat PATE and PATE-F in the epididymis.

<p>Serial sections of the rat tissues were subjected to antigen retrieval in citrate buffer pH 6.0. They were then probed with polyclonal antibodies (1∶250 dilution) raised in rabbit against PATE and PATE-F followed by TRIC (for PATE) or FITC (for PATE-F) conjugated secondary antibody (1∶500 dilution) against rabbit IgG raised in goat. Sections were counter-stained with DAPI. Panels A–C – preimmune serum; D–F – immune serum. Magnification – 10×.