Pharmacological fragmentation of the Golgi induces disruption of the epithelial barrier and disassembly of apical junctions.

<p>Immunofluorescence labeling shows that 24 h incubation of SK-CO15 cells with either Brefeldin A (2 µM) or Golgicide A (50 µM) causes a significant increase in paracellular permeability (<b>A</b>), dramatic disassembly of AJs and TJs (<b>B, arrows</b>), and fragmentation of the Golgi (<b>B, arrowheads</b>); ^#p<0.01 compared to the vehicle-treated group (n = 3). Scale bar, 20 µm.