Epigenetic modifications of Aurora A expression by T1 treatment in breast cancer cells.

<p>A: Effect of the demethylating agent 5′-Azacytine (5-AZA) or the histone deacetylase inhibitor sodium butyrate (SB) treatment on Aurora A gene expression in MCF-7 cells; B: Identification of histone H3 acetylation level of DNA promoter areas in Aurora A gene that are associated with overexpression of Aurora A gene in MCF-7 cells by CHIP; C: Effects of T1 treatment (3<b> </b>µM) on acetylation levels of histone 3 of Aurora A gene by CHIP; D: Effects of SB (1 mM) treatment on the activity of T1 in inhibiting the growth of MCF-7 cells; E: Scheme showing the CHIP primer locations for Aurora A gene. Values were mean±SEM of three independent experiments in triplicates. Within the panel, the value with a letter is significantly different from that of the corresponding control (a, p<0.05; b, p<0.01), and the values with a “*” are significantly different (*, P<0.05).