Site-directed mutagenesis of predicted epitope on prM-E protein.

<p>(A) To confirm the binding epitope of D29 Fab-IgG, residues within the P3 and P9 predicted sequences were mutated to generate mutants 1–6 (M1-6); M3/4, M3/5, M4/5 and M5/6 contain combinational-mutations as stated. (B) Reactivity of antibodies with the mutants was tested by Western blot analysis. Cleared lysate of DENV2-infected Vero cells (DV2), pCMV-prM-E- (prM-E) or mutants-transfected HEK 293 T cells (M1-5/6) were separated on 12% SDS-PAGE in non-reducing condition, followed by detection with h4G2, m2H2 and D29 Fab-IgG.