Aberrant expression of HDAC1 in human HCCs and liver cancer cell lines.

<p>(A) HDAC1 mRNA expression shows gradual increase from pre-cancer to the overt HCCs (*p<0.05). LGDN, low-grade dysplastic nodule; HGDN, high-grade dysplastic nodule; G1–3, Edmondson grades 1–3. (B) Ten pairs of tumor (T) and their corresponding non-tumor (N) liver tissues were obtained from surgical resections. The protein level of HDAC1 was assessed by Western blot analysis. (C) The endogenous levels of HDAC1 in ten liver cancer and three normal cell lines. An antibody against GAPDH served as loading control. (D) Targeted-disruption of HDAC1 causes the growth retardation of HCC cell lines. Cell viability was determined by MTT assay in the indicated cell lines transfected with either scramble (si-Scr) or HDAC1 siRNA (si-HDAC1). Data were expressed as mean ± SD (*p<0.01). All measurements were performed in triplicate, and each experiment was repeated at least two times.