Amino acidic sequence conservation in hemagglutinin groups and subtypes at the region bound by PN-SIA49.

<p>Circles below residues indicate PN-SIA49 percentage binding to each HA alanine mutant compared to binding to the wild-type HA: red 25% binding; yellow 50–75% binding, blue 100% binding. Sequence numbering is based on H1N1 A/Puerto Rico/8/1934 coding region (GenBank accession number ABO21709). Subtypes that can be neutralized by PN-SIA49 are indicated with a green ‘+’ on the left, while the ones that can not be neutralized are indicate with a red ‘−’. ^a Recombinant HA from H1N1 A/South Carolina/1/1918 pandemic strain was previously shown to be bound by PN-SIA49 <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034415#pone.0034415-Burioni1" target="_blank">[26]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034415#pone.0034415-Burioni2" target="_blank">[27]</a>. ^b H1N1 A/New Caledonia/20/1999 was previously shown to be neutralized by PN-SIA49 as Fab fragment <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034415#pone.0034415-Burioni1" target="_blank">[26]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034415#pone.0034415-Burioni2" target="_blank">[27]</a>.