Wound healing in <i>TGM3^−/−</i> skin.

<p>The rate of wound closure in <i>TGM3</i>^−/− mice was not different compared to control animals (A) (n = 9), despite an increase in TGase 3 expression in healing epidermis (B). At day 9 post wounding the thickness of the epidermal layer in the wounded region was greater in wild type skin (C, E) when compared to mutant skin (D, F). Staining of healing skin with keratinocyte differentiation markers in wild type (G, J, M) and <i>TGM3^−/−</i> (H, I, K, L, N, O) animals demonstrated that while there was no change in the expression pattern of the keratinocyte markers loricrin (G–I) or keratin10 (J–L) between the mouse lines, keratin14 (M–O) already showed a more restricted staining pattern reminiscent of unwounded skin. (scale bars C, D 400 µm, E–G 100 µm)