QTL and fine-scale mapping of female fecundity localizes to 5 positional candidate genes.

<p>(A) QTL scan results for fecundity. The <i>x</i>-axis represents position along the three major <i>D. melanogaster</i> chromosomes in megabases (Mb). Tick marks represent location of polymorphic markers used for QTL mapping and black squares represent the approximate location of the centromeres. The <i>y</i>-axis represents the strength of association between a particular region and fecundity. The horizontal line represents the 95% permutation threshold. (B) Deficiency map of the QTL region on 2R. The <i>x-axis</i> represents position along chromosome 2R in megabases (Mb). Tick marks represent location of known genes and the horizontal bars represent the location (either molecularly defined or approximate) of deficiency break points. Grey bars represent deficiencies that complemented the RIL alleles, black bars represent deficiencies that failed to complement the RIL alleles. The five named genes are those genes identified by quantitative complementation as candidates genes affecting fecundity. (C) Estimated effect of the two RIL alleles at the QTL identified on chromosome 2R. Black and white circles represent high and low fecundity alleles, respectively. The <i>x</i>-axis represents larval rearing condition. The <i>y</i>-axis represents estimated fecundity. Error bars represent 95% CI calculated from amongst line variance. (D–E) Results from quantitative complementation tests with the two deficiencies that failed to complement the two alleles in the mapping population. The <i>x</i>-axis of each inset represents the tester chromosome (either “wild-type”- CyO, or deficiency – Def). Black vs. white circles represent the high and low fecundity RIL alleles, respectively. The <i>y</i>-axis of each inset represents estimated fecundity (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002631#s3" target="_blank">Materials and methods</a> for more details). Error bars represent 95% CI based on non-parametric bootstrap resampling (5000 replicates), conditional on fly.