Antibodies to recombinant gingipain in <i>P. gingivalis</i> immunized mice.

<p>Recombinant proteins of the arginine specific gingipain protease of <i>P. gingivalis</i> were produced in <i>E.coli</i>: two proteins in the hemagglutinin/adhesion domain, Rgp44 and Rgp15–27, and the catalytic domain, RgpCAT, which were used in chemiluminescence immunoassay to determine mouse plasma (1∶500) IgM and IgG binding in <i>P. gingivalis</i> immunized (Pg) and control (Co) groups in both A) C57BL/6 and B) LDLR^−/− mice. A) For C57BL/6 immunized and control mice the samples (n = 8 each) were determined as duplicate before (pre) and after (post) immunization. Box-plots represent the distribution of the means of the sample duplicates. B) Two plasma samples within the group were pooled for immunized (black bars) and control (hatched bars) LDLR^−/− mice and measured in duplicate. Samples were collected before (pre), after the second booster immunization (imm) and at the end of HFD (end). Columns represent the mean ± SD of pooled samples in each group. **P<0.01 and *P<0.05.