PTP interacts with ORF1629 in BmNPV-infected cells.

<p>(A) A yeast two-hybrid screen was performed to identify interactions between BmNPV PTP and proteins in BmNPV-infected (12 h p.i.) BmN cells or in epidermal tissues from BmNPV-infected (2 d p.i.) 5^th instar <i>B. mori</i>. This screening identified 5 PTP-interacting clones (12h-3, 12h-11, 12h-4, 12h-16, and 2d-2) by X-gal and 3-AT assays. Clones 12h-3, 12h-11, 12h-4, and 12h-16 were derived from BmNPV-infected BmN cells whereas clone 2d-2 was derived from BmNPV-infected larval <i>B. mori</i>. A legend showing the location of positive standards and PTP-interacting clones (streaked in triplicate) is shown to the right. (B) Interaction of PTP and ORF1629 in BmNPV-infected BmN cells. BmN cells were inoculated with BmNPV (WT) or BmPTPD-wt (D-wt) at an MOI of 5 or mock-infected (mock). BmPTPD-wt expresses FLAG-tagged PTP under an authentic <i>ptp</i> promoter. At 72 h p.i., the cells were harvested and immunoprecipitated with anti-FLAG antibody, and then subjected to western blot analysis (left panels) with anti-ORF1629 antibody or anti-FLAG antibody. The right “Input” panels show western blot analysis using whole cell extracts.