HIV-1 D3 vector analysis.

<p>A) Control, GM-CSF-treated and <i>Leishmania</i>-infected cells were transduced with HIV-1 D3 GFP vector. Twenty-four hours after transduction, the cells were examined for GFP expression (top row). Very few monocytes were GFP+, whereas more cells were GFP+ for the GM-CSF-treated and <i>Leishmania</i>-infected groups. For this experiment, the <i>L. major</i> were labeled with PKH dye and showed that the monocytes were infected. Bright field images were captured for the different groups. B) FACS analysis was done of three different cell populations. Data is representative of four different donors done at 24 h. Complete data sets for 24 h and 48 h are shown in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1002635#ppat-1002635-t001" target="_blank">Table 1</a>.