The species tree of 23 mammals with Old World fruit bats specific amino acid replacements were highlighted by ancestral sequence reconstruction using maximum parsimony method.

<p>(a) A164S, (b) V386I. Branch lengths are not drawn to scale. The amino acids at positions 164 (A: Ala; S: Ser) and 386 (V: Val; I: Ile) of <i>Slc2a4</i> for each interior and exterior node are shown.