Effects of Sal B on rat marrow stromal cell (rMSC) differentiation into osteoblast.

<p>Cells were collected from the femur marrow of one month old SD rats. Cells inoculated in 25 mm² culture flask and cultured. The cells used in the study were the 3 passage, then transferred to the medium containing osteoblast induction medium (OB-in), Sal B and OB-in plus various concentrations of Sal B respectively. A: ALP activity (represent by OD value) was determined at 3, 5 and 7 days. B: the content of osteocalcin (µg/L) in culture medium was determined 1n day 21 after incubated with different treatment. Data shown are mean ± SD. n = 6. **P<0.01, *P<0.05 versus control (vehicle treatment).