Effects of Sal B on the gene expression of Dkk-1/β-catenin pathway in rat marrow stromal cell (rMSC) differentiation.

<p>rMSC were cultured with osteoblast induction medium (OB-in), adipocyte induction medium (Ad-in, i.e. high concentration of GC), with or without Sal B (10-7 mol/L) and Sal A (5×10-7 mol/L, a derivant of Sal B, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034647#pone-0034647-g001" target="_blank">Figure 1</a>) for 7 days. Expression levels of Runx2 (A) , PPARγ (B), β-catenin (C and D) and DKK-1 (E) were measured by RT-PCR. Sal B increased Runx2 and β-catenin mRNA expression and decreased DKK-1 mRNA expression which was similar to the action of OB-in. Ad-in marked increased PPARγ and DKK-1 mRNA expression. When treated the Ad-in rMSC with Sal B, the PPARγ and DKK-1 mRNA expression decreased obviously. Sal A had similar effects to Sal B.