Generation of mice carrying insertion of <i>iCreERT2</i> into the <i>ATG</i> of <i>Upk2</i> gene.

<p><b>A)</b> The BAC clone bMQ-343M5 contains the <i>Upk2</i> gene flanked by parts of <i>Foxr1</i> and <i>Blr1</i> genes, which together with <i>Upk2</i> gene are all transcribed from reverse strand, and <i>Bcl9l</i>, which is from forward strand. The size of the BAC clone insert is 82951 bp (from 44267673 to 44184722 in mouse chromosome 9). <i>iCreERT2-pA</i> is inserted into the <i>ATG</i> start of <i>Upk2</i> gene. ScaI sites and probes for southern blot (P5) are shown. Arrows indicate primers used for PCR genotyping. <b>B)</b> Mouse genotyping by PCR (upper panel) and Southern blot (lower panel). Positive founder lines (#1 and #5) are marked with circles. <i>UIC</i> stands for the <i>TgUICBAC</i> transgene.