ERK is involved in CCL5-mediated migration in human osteosarcoma cells.

<p>(A) MG63 cells were incubated with CCL5 for indicated time intervals, and p-ERK expression was determined by Western blotting. (B) MG63 cells were incubated with CCL5 for indicated time intervals, and ERK kinase activity was examined by ERK kinase assay kit. (C–E) MG63 or U20S cells were transfected with dominant negative (DN) mutant of ERK2 for 24 h followed by stimulation with CCL5. The <i>in vitro</i> migration and integrin expression was examined by Transwell, qPCR, and flow cytometry analysis. Results of five independent experiments performed are expressed. Results are expressed as the mean ± S.E. *, p<0.05 compared with control; #, p<0.05 compared with CCL5-treated group.