Spine density of hippocampal CA1 neurons in sham rat (Control) or adrenalectomized rat (ADX).

<p>(A) Total spine density. For both sham and ADX rats, slices are incubated for 1 h in ACSF without drugs. The Total spine density was not different significantly between Control and ADX rats. (B) Histogram of spine head diameters. Abbreviations are same as in (A). After a 1 h treatment in ACSF without drugs for sham rat (Control, open circle) and ADX (closed black circle). (C) Density of three subtypes of spines. From left to right, small-head spines (small), middle-head spines (middle), and large-head spines (large). Abbreviations are same as in (A). Control (open column) and ADX (black column). Vertical axis is the average number of spines per 1 µm of dendrite. In (A) and (C) results are reported as mean ± SEM. The significance was examined using the Tukey–Kramer <i>post hoc</i> multiple comparisons test when one way ANOVA tests yielded <i>P</i><0.05. For sham and ADX rats, we investigated 3 rats, 6 slices, 12 neurons, 24 dendrites and approx. 1300 spines.