3D morphological analysis of the astrocytic network after iv injection of SRB.

<p><b>A</b>) A multistacks mosaic acquired in the somatosensory area of a coronal acute brain slice (P17 rat). The region of interest (ROI; white rectangle) shows colored astrocytes detected using ImageJ plugins. Each color corresponds to single astrocytes cell body. Scale bar = 150 µm. <b>B</b>) Frequency histogram showing of astrocyte densities as a function of cortical depth calculated from the ROI described above. Depth was divided in 15 bins with 100 µm increments. <b>C</b>) Frequency histogram of normalized astrocytes radial densities in the cortical layer 1 and <b>E</b>) in layers 2/3. <b>D</b>) TPLSM images showing astrocytes in the cortical layer 1 and <b>F</b>) in layers 2/3. Scale bar = 25 µm.