Characterization of tumor derived from spheroid cells.

<p>(A) Histology of tumor derived from spheroid cells. The tumor showed some glandular structure (asterisks) with multiple pathologic mitotic figures (arrowheads, inset) (left), high mitotic rates (arrowheads in middle), and hypervascularization (right) by HE staining. Scale bars: 100 µm. (B) Lung metastasis after tail vein injection of spheroid cells. Lungs were occupied by metastatic tumor nodules. (C) The metastases showed some glandular structure (asterisks) with multiple pathologic mitotic figures (arrowheads in top left); hypervascularization (top right); invasion into lung parenchymal tissue (bottom left) by HE staining. The expression of GFP (Rabbit polyclonal antibody, brown) was found in these metastatic nodules by IHC staining (bottom right). T, tumor; L, lung tissue. Scale bars: 100 µm. (D) Immunohistochemistry of CK and GFP localization in tumors derived from miPS, miPS-LLCcm and spheroid cells. Serial sections were stained with CK (mouse monoclonal antibody, brown) and GFP (Rabbit polyclonal antibody, brown), and counterstained with hematoxylin. Glandular region were CK positive but GFP negative in the tumors. Scale bars: 100 µm.