Progesterone regulation of Jam2 in the ovariectomized mouse uteri.

<p>(A) In situ hybridization of Jam2 mRNA expression after treatment with Oil (control), estrodiol-17β (E2), progesterone (P4) or a combination of estrogen and progesterone (E+P) for 24 h. (B) Immunostaining of JAM2 protein after treatment with steroid hormones for 24 h. (C) Real-time RT-PCR of Jam2 mRNA expression in uteri after ovariectomized mice were treated with Oil (control), estrodiol-17β (E2), progesterone (P4) or a combination of estrogen and progesterone (E+P) for 24 h. (D) Real-time RT-PCR of Jam2 mRNA expression after ovariectomized mice were treated with progesterone for 1, 3, 6, 12, 24 and 48 h, respectively. (E) A representative Western blot of Jam2 protein after ovariectomized mice were treated with progesterone for 6, 12 and 24 h, respectively. (F) The quantitative data in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034325#pone-0034325-g002" target="_blank">Fig. 2F</a>. (G) Real time RT-PCR of Jam2 mRNA expression after ovariectomized mice were treated with progesterone alone or progesterone plus RU486 for 12 h. Sesame oil was served as control. (H) A representative Western blot of JAM2 protein after ovariectomized mice were with progesterone alone or progesterone plus RU486 for 12 h. P, progesterone; Oil, sesame oil. (I) The quantitative data in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034325#pone-0034325-g002" target="_blank">Fig. 2H</a>.