Endotoxin levels in plasma and liver tissues after administration of LPS.

<p>Time course of endotoxin levels in liver (circle) and plasma (square) of BDL (closed) or sham operation (open). (A) Endotoxin was assayed using polychrome LAL kits.* <i>P</i><0.005 (Sham vs BDL group). (B) Immunohistochemical staining for endotoxin in paraffin-embedded liver tissue sections among BDL and sham-operated rats after 0.5 mg/kg administration for 3 h and 24 h. Liver sections were stained using a monoclonal antibody against lipid A (HM2046) and counterstained with hematoxylin. Lipid-A immunoreactivity was detected in hepatocytes (arrowhead) and biliary epithelial cells (arrow), Original magnification: ×200.