<p>AS: PCR amplification success (%); SS: sequencing success (%); Q: percentage of bases with QV (quality value) >20.</p
<p>Equal amounts of cDNAs from all tested samples were mixed as the template. 2.5% non-denaturing ag...
Background: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination po...
<p>Primer sequences used for the validation by qPCR amplification of different genes.</p
Primer sequences for quantitative RT-PCR, ChIP-PCR, and genotyping in the present study.</p
<p>Sequences of qRT-PCR primers, amplicon lengths (bp), PCR efficiencies (%) and correlation coeffic...
<p>Sequences and amplicon characteristics of qRT-PCR primers for nine candidate reference genes and ...
<p>Primer sequences and amplicon characteristics of 17 candidate reference genes for RT-qPCR analysi...
<p>qRT-PCR primer sequences, concentrations and accession numbers for target genes that were designe...
<p>Primer sequences for real-time quantitative reverse transcription-polymerase chain reaction (Q-PC...
<p>Primers used for amplifying coding region of the respective genes by quantitative PCR.</p
*<p>Amplified gene designated by letter before underscore; F and R after underscore indicate forward...
<p>PCR conditions and primers used for the amplification of the ribosomal loci.</p
<p>Primers, amplicon sizes, and qPCR efficiency for the candidate internal control and results valid...
<p>Primer and probe sequences used in the quantitative PCR assays developed in this study.</p
<p>CD4, cluster of differentiation 4; IL17a, interleukin 17a; IFNγ, interferon gamma; PCNA, prolifer...
<p>Equal amounts of cDNAs from all tested samples were mixed as the template. 2.5% non-denaturing ag...
Background: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination po...
<p>Primer sequences used for the validation by qPCR amplification of different genes.</p
Primer sequences for quantitative RT-PCR, ChIP-PCR, and genotyping in the present study.</p
<p>Sequences of qRT-PCR primers, amplicon lengths (bp), PCR efficiencies (%) and correlation coeffic...
<p>Sequences and amplicon characteristics of qRT-PCR primers for nine candidate reference genes and ...
<p>Primer sequences and amplicon characteristics of 17 candidate reference genes for RT-qPCR analysi...
<p>qRT-PCR primer sequences, concentrations and accession numbers for target genes that were designe...
<p>Primer sequences for real-time quantitative reverse transcription-polymerase chain reaction (Q-PC...
<p>Primers used for amplifying coding region of the respective genes by quantitative PCR.</p
*<p>Amplified gene designated by letter before underscore; F and R after underscore indicate forward...
<p>PCR conditions and primers used for the amplification of the ribosomal loci.</p
<p>Primers, amplicon sizes, and qPCR efficiency for the candidate internal control and results valid...
<p>Primer and probe sequences used in the quantitative PCR assays developed in this study.</p
<p>CD4, cluster of differentiation 4; IL17a, interleukin 17a; IFNγ, interferon gamma; PCNA, prolifer...
<p>Equal amounts of cDNAs from all tested samples were mixed as the template. 2.5% non-denaturing ag...
Background: Robust designs of PCR-based molecular diagnostic assays rely on the discrimination po...
<p>Primer sequences used for the validation by qPCR amplification of different genes.</p