Comparison of I3C and DIM in multiple human T-ALL cell lines.

<p>Human CEM, HSB2, SUP-T1 and Jurkat cells were treated for 48 hr with I3C (15.6 up to 500 µM) or DIM (1.9 up to 60 µM), then stained with ViaCount reagent for analysis of cell concentration and percent viability. Values are the mean level of cell proliferation (panels A–D) or the mean percent viable cells (panels E–F) ± SEM (n = 3 independent experiments), normalized to the time-matched vehicle control (0.1% DMSO). Non-linear regression analysis (four parameter, variable slope) was performed (GraphPad Prism) to generate the concentration-response curve for each chemical in each cell line, from which IC₅₀ values were obtained (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034975#pone-0034975-t002" target="_blank">Table 2</a>).