Prior encounter with adenovirus does not inhibit the induction of a transgene- specific CD8 T cell response by the same vector in B cell deficient mice.

<p>B cell deficient mice were vaccinated with Ad5-IiOva followed by a second vaccination with Ad5-IiGP or Ad5-IiNP on day 40. The group receiving Ad5-IiOva followed by Ad5-IiNP received a third vaccination with Ad5-IiGP 40 days after the second immunization. The GP- or Ad5-specific CD8 T cell response was measured on day 11 after the final vaccination (second or third). The experimental design and timeline is outlined above.